한국치위생학회

Antibacterial effect of electrolyzed water on Streptococcus mutans

Ji-Hye Kim1   Ha-Young Youn1   Eun-Kyong Kim4   Young-Eun Lee2   Ji-Eon Jang3   Keun-Bae Song1,*   

Department of Preventive Dentistry, School of Dentistry, Kyungpook National University
1Department of Dental Hygiene, Daegu Health College
2Department of Dental Hygiene, Suseong University
3Department of Dental Hygiene, College of Science & Technology, Kyungpook National University

Abstract

Objectives: Electrolyzed water has been proven to have antibacterial effects against various microorganisms. However, there are only a few studies about effects of electrolyzed water on oral bacteria. The purpose of this study was to examine the antibacterial effect of electrolyzed water on Streptococcus mutans in vitro. Methods: S. mutans KCOM 1054 was treated with electrolyzed water for 1 or 3 minutes and plated on Mitis Salivarius agar with 15% sucrose and bacitracin. After incubation for 48 hours, colony forming units (CFU) were counted, and dental plaque was quantified by crystal violet staining. Results: The growth of S. mutans was significantly inhibited by electrolyzed water (p<0.001). In addition, the dental plaque formation by S. mutans was decreased in a time-dependent manner by exposure to electrolyzed water (p<0.001). Conclusions: Our results suggest that electrolyzed water can effectively prevent dental caries by inhibiting growth of (and the formation of dental plaque by) S. mutans.

Figures & Tables

Fig. 1. Identification of the number of processed for electrolyzed water in 15% sucrose MSB agar plate (A) Tap water, (B) 1 min treatment of electrolyzed water, (C) 3 min treatment of electrolyzed water, (D) Listerin