Abstract
Objectives: Erigeron bonariensis is a type of Erigeron found throughout the tropical and subtropical areas as one of the perennial plants or pioneer plants. It is known to show detoxifying, antipyretic, and anticancer effects for various cancers. However, there are no reports on the anticancer effect of E. bonariensis on oral cancer cells. In the present study, we investigated the effects of the methanol extract of Erigeron bonariensis (MEEB) on the inhibition of cell growth and induction of apoptosis in mucoepidermoid carcinoma (MEC) cell lines, including the MC3 and YD15 oral cancer cells. Methods: MC3 Cells were treated by dimethyl sulfoxide (DMSO) or methanol extracts of 20 various natural products 20 μg/mL for 48 hours and cell viability were analyzed as Trypan blue exclusion assay. The effects of MEEB treatment on the cell viability of MC3 and YD15 cells, for 48 h, were analyzed by Trypan blue exclusion assay. The anticancer efficacy and apoptosis of oral cancer cell lines were analyzed by western blot analysis. The statistical significance of differences between groups was analyzed by Student’s two-tailed t-test. A value of
Figures & Tables
Fig. 1. The effect of sundry natural products on cell viability in MC3 cells The graph represent of three independent experiments of MC3 cells. Cells were treated by dimethyl sulfoxide (DMSO; vehicle control, White bar) or methanol extracts of 20 various natural products 20 μg/mL for 48 hours (#1-20, Black bar), and cell viability were analyzed as trypan blue exclusion assay (0.4%). The graph is mean±SD. *<0.05, Significance compared with the vehicle control-treated group
