Abstract
Objectives: This study aimed to investigate the cytotoxicity of Nexus RMGIC, an indirect restorative cement, on cell survival rate and reactive oxygen species (ROS) production in periodontal stem cells (PDSCs). Methods: PDSCs were incubated with serially diluted Nexus RMGIC eluates with and without the addition of N-acetyl-cysteine (NAC). Cell survival was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The ROS generation was determined by measuring the fluorescence intensity for 2′,7′-dichlorofluorescin diacetate. Results: Nexus RMGIC exposure decreased cell proliferation and cell survival rate in a dose-dependent manner (1:8, 1:4, 1:2, 1:1) in PDSCs. The cytotoxicity of Nexus RMGIC was inhibited by treatment with 10-mM NAC. In addition, the production of ROS was detected by immunofluorescence after PDSCs were exposed to Nexus RMGIC. However, ROS generation was significantly suppressed in the NAC pretreatment compared with the Nexus RMGIC group. Conclusions: Nexus RMGIC increased the cytotoxicity and ROS generation. ROS was involved in Nexus RMGIC-induced cell toxicity.
Figures & Tables
Fig. 1. The cell viability of PDSCs exposed Nexus RMGIC. PDSCs were exposed to different eluates of Nexus RMGIC (1:1-1:8) for 24 h. Representative images are shown in (A). (B) Cell viability of PDSCs exposed to Nexus RMGIC eluates analyzed by WST assay. Nexus RMGIC decreased cell viability of PDSCs in a dose-dependent manner. Data indicated as percentage relative to unexposed control group (100%). *<0.05, **<0.01 compared with control group (only medium).
